Silica Gel Powder for Column Chromatography
Product description
Column chromatography is one of the most widely used techniques for the purification and separation of compounds. In this method, the stationary phase is a solid adsorbent, such as silica gel or activated alumina, and the mobile phase is a liquid solvent.
The separation principle is based on the interaction between the adsorbent’s activity and the solvent’s polarity. When a solvent has low polarity and the adsorbent has high activity, compound separation is highly effective. However, if the solvent polarity is high while the adsorbent activity remains high, separation efficiency decreases, leading to less pure results.
Column chromatography remains a reliable and established method for isolating compounds from complex mixtures. The mixture is packed into a column containing the stationary phase, and the mobile phase flows through it under gravity or applied pressure. The stationary phase may be solid or liquid, while the mobile phase is always liquid, using different solvents as required. This technique offers a versatile and effective approach to achieving targeted purification in chemical and pharmaceutical applications.
Properties of Silica Gel for Column Chromatography
Silchem Pharma silica gel features a high surface area, uniform pore size distribution, low trace elements, and batch-to-batch consistency. Its controlled pH and humidity levels ensure optimal performance, while tight particle structure minimizes fines, making it ideal for efficient separation and purification of compounds..
Uses and Applications
Silica gel is a highly versatile adsorbent, valued for its large surface area and excellent separation capabilities. It is widely used to isolate and purify compounds from complex mixtures across multiple industries.
In the pharmaceutical sector, silica gel is essential for purifying active pharmaceutical ingredients (APIs). In biotechnology, it plays a key role in bio-molecule purification, while in cosmetics, nutraceuticals, and herbal extract processing, it ensures product purity and quality. Research laboratories also rely on silica gel for compound characterization and isolation.
We offers high-quality silica gel in a wide range of mesh sizes (30–800 mesh) to suit diverse applications, delivering reliable performance for industrial, research, and specialty needs.
Analytical Chromatography
The collectively term of chromatography is may be analytical or preparative. The starting phase of chromatography is analytical chromatography with little amount of silica gel mesh 60-120 size by using analytical column packaging, to analysis how many percentage of mixture is purify. Analytical chromatography is a simple method of chromatography with faster and cost effective separation. In analytical chemistry development, techniques for solvingchemical subtracts by using thin layer plates coated silica gel on glass plate. This technique becomes standard analytical tools in pharmaceutical laboratories..
Preparative Chromatography
Focus on the latest chromatography technologies such as preparative and process chromatography to optimize the current and standard opportunities to optimize chromatography process in proper way. After preparative process, improving impurity profile and speed of separation use semi-preparative chromatography.
Process Chromatography
Process chromatography technology is utilized on a large scale with dynamic binding capacity. We are developing large scale process chromatography for improving purity in various biologics such as proteins, virus, vitamins, hormones, anti-bodies. In process chromatography use stationery phase adsorbents like silica gel and activated alumina are used in bulk quantity. Silica gel 230-400 and 400-800 mesh sizes are used in process chromatography to get more purity of active compounds.
Gravity Chromatography
Gravity chromatography is a manual process of require continuously observed. Solvent allowed to move down the column by gravitational forced and the flow rate can be manually controlled. The practical size use of silica gel is 70-230 mesh and 63-210 microns.
The different mesh sizes manufactured by us are as follows
- 60/120 mesh. (120-250) micron.
- 100/200 mesh.(75-150) micron.
- 70/230 mesh. (63-200) micron.
- 230/400 mesh. (37-63) micron.
- 70/325 mesh. (45-200) micron.
- 30/200 mesh.
Technical specification
- Grade : Pharma and Laboratory Grade
- EC No. : 231-545-4
- HS Code : 2811 22 00
- Storage : Below 30°C
- Shelf life : 5 years
Parameter
Values
Size in Mesh | 60-120 mesh | 100-200 mesh | 230-400 mesh |
CAS No | 63231-67-4 | 63231-67-4 | 63231-67-4 |
Molecular Formula | SiO2 | SiO2 | SiO2 |
Molecular Weight | 60.08 | 60.08 | 60.08 |
Description | Free flowing white to offwhite odourless powder. | Free flowing white to off-white odourless powder. | Free flowing white to off-white odourless powder. |
Solubility | Insoluble in water, alcohol and acids. | Insoluble in water, alcohol and acids. | Insoluble in water, alcohol and acids. |
Assay(as SiO2) | Min. 99.50% | Min. 99.50% | Min. 99.50% |
Mesh size: | min.95%(60-120mesh) (125 - 250µm) | min.95% (100-200 Mesh) (149-74μm) | min.95% (230-400 Mesh) (40-63 μm) |
Particle size 1 | min. 80.00% passing through 60 mesh (250 µm) | min. 80.00% passing through 100 mesh (149 µm) | min. 80.00% passing through 230 mesh (40 µm) |
Particle size 2 | max. 20.00% passing through 120 mesh (125 µm) | max. 20.00% passing through 200 mesh (74 µm) | max. 20.00% passing through 400 mesh (63 µm) |
Adsorption capacity @ 100% humidity | 30 - 35% w/w | 30 - 35% w/w | 30 - 35% w/w |
Column chromatography test | Passes | Passes | Passes |
pH (10% aqueous suspension at 25°C) | 6.5 - 7.5 | 6.5 - 7.5 | 6.5 - 7.5 |
Loss on drying,130°C | 3.00% max | 3.0% max | 3.0% max |
Chloride(Cl) | 0.1% max | 0.1% max | 0.1% max |
Chloride (as NaCl) | Max 300ppm | Max 300ppm | Max 300ppm |
Fe (Iron): | <75 ppm | <75 ppm | <75 ppm |
Boiling Point | 2230 0C | 2230 0C | 2230 0C |
Melting Point | >1600 0C | >1600 0C/td> | >1600 0C/td> |
Bulk density | 0.5 - 0.6 g/ml | 0.5 - 0.6 g/ml | 0.5 - 0.6 g/ml |
Pore Diameter –A | 40-60A | 40-60A | 55-60A |
Surface Area m2 /gm | 350-450 | 350-550 | 450-650 |
Moisture Content % | <6.00 | <6.00 | <6.00 |
Available pack size
500 GRAM, 5 KG DRUM, 25 KG DRUM